| Title | Prof. | First Name | Arun | Last Name | Jagannath |
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|---|---|---|---|---|---|---|---|
| Designation | Professor | ||||||
| Department | Department of Botany | ||||||
| Webpage | |||||||
| Phone.no | 9999999999 | ||||||
| Employement Info | |||||||
|---|---|---|---|---|---|---|---|
| Employee Type | Nature Of Employment | ||||||
| Teaching | Permanent | ||||||
| Educational | |||||||
|---|---|---|---|---|---|---|---|
| Degree/Certification | Institution | Year | |||||
| Certificate | Indian Institute of Technology, Delhi (Supercomputing Facility for Bioinformatics and Computational Biology) and NIIT | 2004 | |||||
| Ph.D. - Modification and Syntheses of barstar and bar Genes and Development of barnase/barstar Lines in Brassica juncea L. for Hybrid Seed Production | University of Delhi | 1999 | |||||
| PG | Madurai Kamaraj University | 1994 | |||||
| UG | University of Delhi | 1992 | |||||
| Research Projects | |||||||
|---|---|---|---|---|---|---|---|
| Title of the Project | Project Type | Year Of Sanction | Output | Start Date | End Date | ||
| Genotyping by Sequencing of two mapping populations (D and E) of the oilseed crop, Carthamus tinctorius (Safflower) for mapping of agronomic traits | 2022 | Genomic DNA was isolated for populations D and E. GBS libraries were prepared with PstI restriction enzyme and issues related to library quality and purity were resolved. High-throughput sequencing of these libraries is in progress. Pipelines for GBS data analysis and construction of linkage maps were optimized in the laboratory. These pipelines will be utilized for SNP calling and construction of a high-density linkage map on receipt of the GBS data. Phenotypic data for traits of agronomic value for the two populations were collected from three independent locations in India. These would be used for QTL mapping. Loci thus identified will facilitate development of effective breeding programs for crop improvement. | 2022-08-31 | 2023-02-15 | |||
| Transcriptome sequencing of Lipaphis erysimi and Myzus persicae and analysis of differentially expressed genes between the two aphid species and their developmental stages. | 2021 | Nymphs and adults of both the aphid species were reared inside clip cages attached to the leaves of Brassica juncea var. Varuna growing inside containment net-houses. Nymphal stages i.e. N1,N2, N3, N4 were pooled together and then harvested along with adult aphids. Three biological replicates for each aphid species and developmental stage were harvested after 10-15 days. Total RNA was extracted from aphids using standardized protocols available in our laboratory. cDNA libraries were prepared using NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina®. High-throughput RNA-Seq for 12 cDNA libraries was performed on Illumina platform which generated millions of paired-end reads. These raw reads were filtered to remove adaptor contamination. High quality reads were assembled using Trinity and differentially expression of genes between two aphid species as well as between their developmental stages have been performed. There are 6560 genes which were up-regulated and 1527 genes which were down-regulated in Myzus persciae adult whereas there are 4852 genes which are up-regulated and 7176 genes are down-regulated in Lipaphis erysimi adult when compared to their respective nymph stage transcripts. Final assembly was used to perform annotation and gene ontology. Further analysis and validation of the data is under process. | 2021-10-29 | 2022-03-31 | |||
| Exploiting Genetic Diversity for Improvement of Safflower through Genomics-Assisted Discovery of QTLs/Genes Associated with Agronomic Traits | Major | 2020 | Ongoing | 2020-02-29 | |||